DNA Sample Collection
Once you are committed to carrying out a DNA test at IDENTIGENE, the next step is to decide whether you need a test that will hold up in a court of law. Some individuals are not concerned about this. They may need to determine a genetic relationship only for their own information or to gain peace of mind, and don’t plan to use the test results for any legal proceeding. If this describes your situation, then you may wish to order a
home paternity test collection kit from IDENTIGENE. This
paternity test kit contains all the necessary materials to collect specimens from the parties involved in the test (e.g., the Mother, Child and Alleged Father involved in a paternity test). The home collection kit also includes specimen identification documents to be completed by the tested parties and an express mail envelope in which to send the specimens to our
DNA testing laboratory in Houston, TX. Since it is impossible to certify whose samples we tested in a home
paternity test, the final report for such a test will contain the following disclaimer:
IDENTIGENE expressly disclaims any and all responsibility for the accuracy and validity of this report. The collection of specimens for the performance of the paternity testing and analysis that forms the basis for this report was not in compliance with IDENTIGENE's established chain of custody guidelines, thus the results of this report can not be considered to be a scientifically accepted paternity test for use in a court of law.
If the DNA test you wish to perform must be legally binding, you should
choose to have a legal paternity test performed. If DNA test results are needed
for legal purposes, it is necessary that all samples be collected in a fashion that insures a documented “chain of custody.” By this we mean that, in order for IDENTIGENE to issue a report acceptable in a court of law, we must have a method for collecting and testing
DNA specimens that enables us to certify that we are reporting genetic test results for the correct individuals. To obtain legally-binding
paternity test results, you must arrange to have all specimens collected by an IDENTIGENE specimen collector. This can easily be arranged on-line by completing a request form for a “legally-binding” paternity test (either Trio or Motherless). An IDENTIGENE Case Manager will call you on the next business day to arrange for collection of specimens at a place and time convenient for you.
A Brief History
DNA
Paternity testing was performed in the middle half of the twentieth century by comparing blood types of tested parties. This involved isolation of blood sera from antigen-challenged individuals that did not possess certain red blood cell antigens. These antigens are protein molecules that may be complexed with sugar molecules and reside in the red blood cell membrane. These sera cause coagulation of red blood cells in individuals that possess that particular red blood cell antigen. For example, in the well-known ABO system, humans can possess the A antigen (A blood type), the B antigen (B blood type), both the A and B antigen (AB blood type), or neither of these antigens (O blood type). Red blood cell antigen systems of this sort can be used for paternity testing because there are genes that code for the antigens and we inherit those genes from our parents.
So, for example, a mother who has Type A blood and a father who has Type O blood could not have a child who has type AB blood. The true father of the child must have the gene for the B antigen. Using RBC antigen systems for paternity testing did not provide for a very powerful test because the frequencies of the genes that coded for the antigens are not very low. Eventually, in the late 1970’s a more powerful test using white blood cell antigens or Human Leukocyte Antigens (HLA) was developed and added to batteries of RBC antigen tests. This produced a test that was able to exclude about 95% of falsely accused fathers. This pales in comparison to most DNA test batteries presently used for paternity analysis (exclusion powers ranging from 99.9% to 99.9998%). One other requirement for this type of testing is that lots of blood (several milliliters) is required to perform the test. Even the next generation of testing, DNA testing by Restriction Fragment Length Polymorphism (RFLP) analysis, requires a fairly substantial amount (3-5 mls) of blood.
The use of blood for paternity testing is firmly entrenched historically. However, if the analysis is performed strictly by DNA testing, there is no need to obtain the DNA specimen from blood cells (only the white blood cells contain nuclear DNA, by the way, because red blood cells possess no nucleus). All the nucleated cells in your body contain the same DNA, so no matter where on your body we obtain the DNA, we will observe the exact same genotype.
IDENTIGENE Process
We utilize a process called the Polymerase Chain Reaction (PCR) to amplify the genes we are typing (see Amplification of STR Loci),
so we do not need very much starting material. Because of this, we have chosen a very quick, painless, and easy process for
DNA specimen collection: buccal swabs. These are collected by simply rubbing a sterile cotton-tipped swab on the inside of your cheek. We collect two swabs from each individual so that we have a method of specimen verification, if necessary, or if one swab fails to produce results.
The reason that cheek swabs make excellent specimens for
DNA testing is that epithelial cells are constantly being shed from the mucosal layer of the inside of your mouth. In fact, on one correctly collected swab, we obtain close to a million of these cells.
Once the cells have been swabbed from the inside of your mouth, they stick to the cotton tips of the swabs. These swabs are then air-dried which lyses the cells. The dried swabs are packaged in specimen envelopes that contain all necessary identification for the collected individual (Name, Date of Birth, Race, Party in Test). If the specimens are collected by a certified IDENTIGENE specimen collector s/he will also have collected individuals sign their specimen envelope to verify it as their own as well as initial it her/himself to validate the legal chain of custody for the specimens. He/she will also complete an IDENTIGENE Client Authorization form that provides us with the client’s authorization to perform the testing as well as a means of identifying the tested parties by photographs and signatures.
Specimens collected using home paternity test kits will not, of course, possess this legal chain of custody. The results from these
DNA tests will be issued on reports containing the following disclaimer:
IDENTIGENE expressly disclaims any and all responsibility for the accuracy and validity of this report. The collection of specimens for the performance of the paternity testing and analysis that forms the basis for this report was not in compliance with IDENTIGENE's established chain of custody guidelines, thus the results of this report can not be considered to be a scientifically accepted paternity test for use in a court of law.
Sample Collection
Sample Shipment
Sample Login
DNA Extraction
DNA Amplification
Laser Detection
Result Analysis
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